Open AccessUltrastructural aspects of fragility of Pseudomonas aeruginosa outer membrane devoid of protein F
Author(s) -
Gotoh Naomasa,
Wakebe Hirokazu,
Nishino Takeshi
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03081.x
Subject(s) - osmotic shock , ultrastructure , pseudomonas aeruginosa , bacterial outer membrane , erythrocyte fragility , inner membrane , osmotic pressure , shock (circulatory) , biology , membrane , cell membrane , electron microscope , biophysics , microbiology and biotechnology , chemistry , biochemistry , bacteria , escherichia coli , anatomy , genetics , immunology , medicine , hemolysis , physics , gene , optics
The protein F‐deficient cells of Pseudomonas aeruginosa were previously found to be more susceptible to osmotic shock than the sufficient cells (Gotoh et al., J. Bacteriol., in press). The protein F‐deficient cells were observed by the thin‐section method of electron microscopy to determine the effects of osmotic shock. The osmotic shock induced breakage of the protein F‐deficient outer membrane, while it had no effect on the protein F‐sufficient outer membrane. These results suggested that the cells lost their viability by the osmotic shock caused by fragility of the outer membrane.