Open AccessPurification and some properties of a novel ethylene‐forming enzyme produced by Penicillium digitatum
Author(s) -
Fukuda Hideo,
Kitajima Hirotake,
Fujii Takao,
Tazaki Masato,
Ogawa Takahira
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03072.x
Subject(s) - penicillium digitatum , ethylene , enzyme , chemistry , penicillium , biochemistry , microbiology and biotechnology , biology , botany , food science , antifungal , catalysis
A novel ethylene‐forming enzyme that forms ethylene from α‐ketoglutarate was purified from the cell‐free extract of Penicillium digitatum IFO 9372. The purified enzyme was confirmed to be homogenous on polyacrylamide disc gel electrophoresis, and the molecular weight of the enzyme consisting of a single polypeptide was estimated to be about 42 kDa by gel filtration column chromatography and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The presence of α‐ketoglutarate, l ‐arginine, ferrous ion, dithiothreitol and oxygen were essential for the enzyme reaction. Substrate and cofactor specificity of the enzyme was highly specific for α‐ketoglutarate and l ‐arginine, respectively. Some properties of the purified enzyme were surveyed and compared with those of the ethylene‐forming system in higher‐plants.