Open AccessRapid identification of medically important yeasts by electrophoretic protein patterns
Author(s) -
Bruneau S.,
Guinet R.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03068.x
Subject(s) - geotrichum , yeast , candida tropicalis , candida krusei , biology , microbiology and biotechnology , candida albicans , corpus albicans , staining , candida parapsilosis , biochemistry , saccharomyces cerevisiae , genetics
A simple electrophoretic method for yeast identification was evaluated. Whole cells were extracted by SDS and the protein profiles obtained in SDS‐PAGE after Coomassie blue staining were compared for 52 strains from 9 species of yeast or yeast‐like fungi commonly isolated from man ( Candida albicans, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, C. pseudotropicalis, C. tropicalis, Geotrichum candidum, Saccharomyces cerevisiae ). The corresponding patterns showed 30 to 45 polypeptides in the range 95‐20 kDa and were clearly different for the 9 species. No differences could be detected between strains from the same species. The characteristic patterns were obtained within 24 h allowing rapid identification of the most commonly encountered clinical yeast isolates.