Open AccessChromosomal mapping of genes encoding mannose‐sensitive (type I) and mannose‐resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5
Author(s) -
KrallmannWenzel Ulrike,
Ott M.,
Hacker J.,
Schmidt G.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03066.x
Subject(s) - fimbria , operon , gene , biology , gene cluster , mannose , escherichia coli , genetics , threonine , chromosomal region , microbiology and biotechnology , chromosome , biochemistry , serine , phosphorylation
DNA hybridization experiments demonstrated that the gene clusters encoding the F8 fimbriae ( fei ) as well as the type I fimbriae ( pil ) exist in a single copy on the chromosome of E. coli O18:K5 strain 2980. In conjugation experiments with appropriate donors, the chromosomal site of these gene clusters was determined. The pil genes were mapped close to the gene clusters thr and leu controlling the biosynthesis of threonine and leucine, respectively. The fei genes were found to be located close to the galactose operon ( gal ) between the position 17 and 21 of the E. coli chromosomal linkage map.