Open AccessComplete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG
Author(s) -
Terasaka Kunihiro,
Yamaguchi Ryuji,
Matsuo Kazuhiro,
Yamazaki Akihiro,
Nagai Sadamu,
Yamada Takeshi
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03058.x
Subject(s) - mycobacterium bovis , microbiology and biotechnology , virology , sequence (biology) , nucleic acid sequence , peptide sequence , nucleotide , biology , mycobacterium tuberculosis , medicine , genetics , gene , tuberculosis , pathology
The extracellular protein MPB70 is a heat‐stable immunogenic protein which was found in the culture filtrate of Mycobacterium bovis BCG Japanese. We determined the complete nt and aa sequences of MPB70 and correlated with the previously reported data. The N‐terminal sequence revealed that the signal peptide (SP) consisted of 30 aa and that the mature protein had 163 aa with a molecular weight of 16 305. The SP displayed a characteristic feature of an Ala‐rich property which would be efficient in a SP function.