Open AccessProtoplast formation and regeneration of a thermophilic Clostridium sp.
Author(s) -
Julia Pettinari M.,
Ivanier Sofía E.,
Méndez Beatriz S.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03054.x
Subject(s) - protoplast , thermophile , regeneration (biology) , biology , microbiology and biotechnology , clostridium , chemistry , bacteria , botany , genetics
Abstract Protoplasts of a thermophilic Clostridium sp. were prepared by lysozyme treatment using lactose as osmotic stabilizer. High frequency reversion (3–29.8%) to the bacillary form was obtained on hypertonic rich medium.