Open AccessA membrane‐bound isobutene‐forming enzyme from Rhodotorula minuta
Author(s) -
Fujii Takao,
Ohga Yoshinobu,
Ogawa Takahira,
Fukuda Hideo
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03044.x
Subject(s) - chemistry , microsome , cytochrome , enzyme , biochemistry , rhodotorula , membrane , stereochemistry , chromatography , organic chemistry , yeast
An Arrhenius plot for the formation of isobutene by cell extracts of Rhodotorula minuta IFO 1102 gave a discontinuous line and isobutene‐forming activity was found to be present in the microsomal fraction of the extracts. These results indicate that the isobutene‐forming enzyme is membrane‐bound. The formation of isobutene by the microsomes was inhibited by some redox reagents and completely eliminated by the presence of carbon monoxide. These findings suggested that a cytochrome P‐450 participated in the reaction. In fact, a cytochrome P‐450 and cytochrome P‐450 reductase were detected in the microsomes obtained from cells that contained the isobutene‐forming activity.