Open AccessTransformation of Bacillus thuringiensis by electroporation
Author(s) -
Bone Eileen J.,
Ellar David J.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03039.x
Subject(s) - bacillus thuringiensis , electroporation , plasmid , transformation (genetics) , bacillaceae , biology , mutant , bacillales , microbiology and biotechnology , escherichia coli , strain (injury) , dna , transformation efficiency , bacteria , gene , genetics , bacillus subtilis , agrobacterium , anatomy
Plasmids were transformed by electroporation into various strains of Bacillus thuringiensis with frequencies of up to 10 5 transformants/μg. pC 194 transformed all strains tested at a high frequency and cells could be stably transformed with pC194 and pUB110 simultaneously by electroporation with a frequency of 10 2 pC194 + pUB110 transformant/μg DNA. Low transformation frequencies observed with some plasmids, especially those grown initially in Escherichia coli , could be increased by passage through B. thuringiensis, B. thuringiensis var. israelensis and an acrystalliferous mutant of the same strain transformed at frequencies of 10 4 –10 5 /μg DNA with most of the plasmids tested. A cloned israelensis 27‐kDa δ‐endotoxin gene was introduced into the israelensis acrystalliferous mutant and a kurstaki acrystalliferous mutant by electroporation. Both transformants were shown to express the endotoxin gene and to be toxic to Aedes aegypti larvae.