Open AccessCloning of the glycerol kinase gene of Bacillus subtilis
Author(s) -
Holmberg Christina,
Rutberg Blanka
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03036.x
Subject(s) - glycerol kinase , bacillus subtilis , plasmid , glycerol , escherichia coli , microbiology and biotechnology , biology , gene , mutant , cloning (programming) , molecular cloning , biochemistry , genetics , bacteria , peptide sequence , computer science , programming language
A 3.5 kb fragment of Bacillus subtilis DNA which contains wild type alleles of mutations in glpK (glycerol kinase) and glpD (glycerol‐3‐phosphate [G3P] dehydrogenase) was cloned in plasmid pHV32 in Escherichia coli . The cloned fragment expresses glycerol kinase in B. subtilis mutants carrying the mutations glpK 11 and recE 4 after induction with glycerol or G3P whereas it does not express G3P dehydrogenase. The cloned fragment thus contains the complete glpK but probably only part of glpD .