Open AccessIdentification of ‘ Campylobacter upsaliensis ’ and other catalase‐negative campylobacters from paediatric blood cultures by numerical analysis of electrophoretic protein patterns
Author(s) -
Owen R.J.,
Morgan D.D.,
Costas M.,
Lastovica A.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03035.x
Subject(s) - campylobacter , campylobacter jejuni , microbiology and biotechnology , catalase , biology , strain (injury) , thermophile , bacteria , enzyme , genetics , biochemistry , anatomy
Twenty‐one strains of catalase‐negative campylobacters from paediatric blood cultures were characterized by one‐dimensional SDS‐PAGE of cellular proteins. A further 11 Campylobacter strains were included for reference purposes. The partial protein patterns were used as the basis of a numerical analysis, which showed that 17 hippurate‐negative strains had a high similarity to ‘ C. upsaliensis ’ ( r ? 0.82) irrespective of their geographical location, and that three hippurate‐positive isolates had a high similarity ( r ? 0.87) to C. jejuni subsp. jejuni . One hippurate‐positive CNW strain was not identified. The analysis of SDS‐PAGE protein patterns proved an excellent method of characterizing these thermophilic campylobacter as they were difficult to identify by traditional methods.