Open AccessA screening for the presence of four different crystal protein gene types in 25 Bacillus thuringiensis strains
Author(s) -
Visser Bert
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03030.x
Subject(s) - bacillus thuringiensis , gene , homology (biology) , biology , serotype , spodoptera , genetics , bacillaceae , homologous chromosome , strain (injury) , coding region , microbiology and biotechnology , bacteria , recombinant dna , anatomy , bacillus subtilis
25 different strains of Bacillus thuringiensis , belonging to 18 different serotypes were screened by Southern hybridization analysis for the presence of nucleotide sequences of four different gene types coding for crystal proteins showing different insecticidal spectra. Many but not all strains showed sequences homologous to any of the probes used. Homology with sequences of the gene type present in strain kurstaki HD1 occurred in all positive strains, whereas the other gene types were much less abundant. Sequences homologous to those of gene type VI, coding for a Spodoptera‐ specific crystal protein, appeared only in strains of serotypes aizawai and entomocidus .