Open AccessLactobacillus lactis protoplasts at different stages of cell cultivation
Author(s) -
Bican Pavel,
Spahni Andreas
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03024.x
Subject(s) - protoplast , lactobacillus , molecular mass , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , biochemistry , chemistry , acrylamide , cell , gel electrophoresis , bacteria , biology , chromatography , enzyme , fermentation , organic chemistry , copolymer , genetics , polymer
Protein profiles of both protoplasts and cell homogenates of Lactobacillus lactis prepared in the course of cell multiplication were compared. The sodium dodecyl sulfate acrylamide gel electrophoretograms of whole cell homogenates and protoplasts show almost the same complexity. Protoplast protein profiles after 4 hours of growth exhibit new bands in both the low and high molecular mass region of the gel. The changes in protein composition during conversion of the cells into protoplasts are predominantly quantitative in nature.