Open AccessMultiple forms of carboxylesterase activity in Acinetobacter calcoaceticus
Author(s) -
Sherwani M.K.,
Fixter L.M.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03021.x
Subject(s) - acinetobacter calcoaceticus , phenylmethylsulfonyl fluoride , carboxylesterase , chemistry , strain (injury) , acinetobacter , specific activity , biochemistry , fluoride , microbiology and biotechnology , enzyme , chromatography , biology , pmsf , antibiotics , anatomy , inorganic chemistry
A carboxylesterase activity (E.C.3.1.1.) was found in all four strains of Acinetobacter calcoaceticus tested. The activity was present in both 2 × 10 4 g av h −1 supernatant and bacterial wall‐membrane fractions. The activity in the supernatant was in two molecular weight forms, the predominant form with a M r of about 10 3 K and a minor form M r approximately 600 K. The activity was inhibited by phenylmethylsulfonyl fluoride. SDS‐PAGE showed that in A. calcoaceticus NCIB 8250 the activity was composed of three components of M r 43, 40 and 38 K. The individual components showed different activities with 1‐ or 2‐naphthyl esters. Of the other strain used, one showed a nearly identical pattern of component activities, while the other two showed only two component activities.