Open AccessOccurrence of Salmonella typhimurium virulence plasmid‐specific sequences in different serovars of Salmonella
Author(s) -
Korpela Kai,
Ranki Marjut,
Sukupolvi Soila,
Mäkelä P.Helena,
Rhen Mikael
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03016.x
Subject(s) - plasmid , virulence , pbr322 , biology , salmonella , homology (biology) , serotype , microbiology and biotechnology , enterobacteriaceae , homologous chromosome , genetics , pathogenicity island , gene , escherichia coli , bacteria
We have subcloned the 96‐kilobasepair (kb) virulence plasmid, pLT2, of Salmonella typhimurium line LT2 into 7 subfragments. Using these subclones as probes, 35 independent Salmonella isolates were tested for complementary DNA sequences. Sequences homologous to pLT2 were present in 15 of the isolates. All of these contained sequences homologous to at least one specific probe representing 15 kb of pLT2. The traT gene from pLT2 was absent in 6 of these 15 isolates. Three strains reported to be cured of the plasmid were shown to harbour some sequences with homology to the pLT2 plasmid. Seven isolates were shown to contain homologous sequences with pBR322 but not with the pLT2 plasmid.