Open AccessConstruction of shuttle vectors useful for transforming Clostridium acetobutylicum
Author(s) -
Truffaut N.,
Hubert J.,
Reysset G.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb03010.x
Subject(s) - shuttle vector , plasmid , clostridium acetobutylicum , pbr322 , replicon , transformation (genetics) , tetracycline , biology , microbiology and biotechnology , escherichia coli , bacillus subtilis , recombinant dna , genetics , dna , gene , vector (molecular biology) , bacteria , antibiotics , biochemistry , butanol , ethanol
Plasmids pIM13, pT127 and pBC16δ1, introduced by transformation into Clostridium acetobutylicum N1‐4081, were shown to replicate in, and to confer antibiotic resistance upon this new host. Recombinant plasmids were contructed by inserting erythromycin‐resistant plasmid pIM13 into the unique Cla I site of pBR322 or by ligating a tetracycline‐resistant determinant of plasmid pT127 to Hin dIII‐linearized pIM13. The hybrid plasmids replicated and expressed erythromycin resistance in C. acetobutylicum strain N1‐4081 and in Escherichia coli or Bacillus subtilis , indicating that they might be useful as shuttle vectors for transferring genes between these strains. The efficiency and stability of different replicons in C. acetobutylicum were compared.