Open AccessRestriction endonuclease analysis of four Borrelia burgdorferi strains
Author(s) -
Cinco M.,
Banfi E.,
Balanzin D.,
Cacciò S.,
Graziosi G.,
Fattorini P.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb02443.x
Subject(s) - restriction enzyme , borrelia burgdorferi , hindiii , restriction site , biology , virology , microbiology and biotechnology , restriction digest , ecori , genetics , restriction map , dna , plasmid , antibody
A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi : one isolated from man (SF), one from Ixodes dammini (B31) and two form I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies.