Open AccessOptimal conditions for the toxoiding of pertussis toxin with 1‐ethyl‐3(3‐dimethylaminopropyl) carbodiimide · HCl
Author(s) -
Christodoulides M.,
Parton R.,
StewartTull D.E.S.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb02434.x
Subject(s) - carbodiimide , antigenicity , pertussis toxin , histamine , toxoid , chemistry , pertussis vaccine , microbiology and biotechnology , chromatography , antigen , biology , pharmacology , biochemistry , immunology , immunization , g protein , receptor
The optimal conditions for toxoiding a pertussis toxin (PT) preparation with 1‐ethyl‐3(3‐dimethylaminopropyl) carbodiimide · HCl (EDAC) were determined. The prime factor affecting the toxoiding of PT was the EDAC to protein ratio. A ratio of 40–80 : 1 EDAC to protein by weight was optimal for abolishing the acute toxicity, histamine‐sensitising and leucocytosis‐promoting activities associated with PT, whilst maintaining the antigenicity of the vaccine antigens. An EDAC‐toxoid also manifested no late histamine‐sensitising activity. Duration of exposure to EDAC, temperature and pH value of the reaction were found not be be critical for toxoiding. The data indicated that the use of EDAC for toxoiding PT in a B. pertussis extract is a simple and reproducible procedure and should be considered as a method for the production of acellular pertussis vaccines.