Open AccessImmunoprotective extracellular polysaccharides of Klebsiella aerogenes capsular type K1, expressed in Escherichia coli
Author(s) -
Roe E.A.,
Jones R.J.,
Saunders J.R.
Publication year - 1989
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1989.tb02390.x
Subject(s) - enterobacter aerogenes , escherichia coli , microbiology and biotechnology , extracellular , biology , klebsiella , polysaccharide , immunodiffusion , extracellular polysaccharide , enterobacteriaceae , biochemistry , gene , antibody , genetics
A gene library of genomic DNA Klebsiella aerogenes of capsular serotype K1 was constructed in E. coli LE392 using the cosmid pMMB33. Culture filtrates of E. coli recombinants were screened by ELISA for extracellular polysaccharides specific for K. aerogenes K1. Extracellular polysaccharide extracts from K. aerogenes K1 and 3% of the E. coli recombinants contained immunoprotective extracellular polysaccharides (IEP) with similar chemical and immunological properties as shown by gel filtration through Sephacryl 1000, double immunodiffusion and mouse protection tests. IEPs contained no detectable protein, had molecular weights of several hundred million and protected mice against lethal autologous K. aerogenes K1 challenge at a dosage of 10 nanograms per mouse.