Open AccessNucleotide and deduced amino acid sequence of the Rhodobacter sphaeroides gene encoding form II ribulose‐1,5‐bisphosphate carboxylase/oxygenase and comparison with other deduced form I and II sequences
Author(s) -
Wagner Stephen J.,
Edward Stevens S.,
Tracey Nixon B.,
Lambert David H.,
Quivey Robert G.,
Robert Tabita F.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb13937.x
Subject(s) - rhodobacter sphaeroides , ribulose 1,5 bisphosphate , rhodospirillum rubrum , rhodobacter , oxygenase , biochemistry , ribulose , biology , nucleic acid sequence , pyruvate carboxylase , rubisco , peptide sequence , gene , enzyme , genetics , photosynthesis , mutant
The nucleotide sequence for the Rhodobacter sphaeroides form II ribulose 1,5‐bisphosphate carboxylase/oxygenase was determined. The deduced product is highly homologous with the form II‐like enzyme of Rhodospirillum rubrum , but appears to be more distantly related to the large subunit of the L 8 S 8 enzyme found in autotrophic bacteria, cyanobacteria and higher plants. Several regions highly conserved among L 8 S 8 and L X enzymes correspond with regions previously implicated in catalytic activity and subunit interactions. An imperfect palindrome and a stem loop structure were identified in the 5′ and 3′ flanking sequences, respectively, of R. sphaeroides rbpL .