Open AccessPhysiological overlap between osmotolerance and thermotolerance in Saccharomyces cerevisiae
Author(s) -
Trollmo Christina,
André Lars,
Blomberg Anders,
Adler Lennart
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03200.x
Subject(s) - osmotic shock , saccharomyces cerevisiae , glycerol , osmotic pressure , heat shock protein , biochemistry , biology , dehydration , enzyme , yeast , chemistry , gene
Exponentially growing cells (23°C) of Saccharomyces cerevisiae were conditioned to heat (37°C) or to osmotic dehydration (0.7 M NaCl) for a period of one hour, whereafter thermo‐ and osmotolerance of the cells were studied. Thermotolerance was measured as survival at 47°C, while osmotolerance was determined as the capacity of the culture to form colonies on 1.5 M NaCl medium. Cells conditioned to osmotic dehydration were shown to be as thermotolerant as heat conditioned cells; the death rate constant being 20‐fold lower than for non‐conditioned cells. Heat conditioned, cells, however, were not osmotolerant. The physiological overlap between osmotolerance and thermotolerance was thus unidirectional. The enzyme sn ‐glycerol 3‐P dehydrogenase (GPDH) (EC 1.1.1.8), was induced by osmotic stress, but was not induced upon exposure to heat. Thus GPDH was shown not to be a heat shock protein.