Open AccessIsolation and characterization of hemolysin mutants of Vibrio vulnificus
Author(s) -
Massad George,
Simpson Linda M.,
Oliver J.D.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03195.x
Subject(s) - virulence , hemolysin , vibrio vulnificus , cytolysin , microbiology and biotechnology , biology , mutant , hemolysis , pathogen , vibrionaceae , bacteria , gene , genetics , immunology
The importance of the cytolysin/hemolysin in the virulence of Vibrio vulnificus was investigated using both the naturally occuring virulent and avirulent colony variants and ethylmethane‐sulfonate generated mutants. Both virulent and avirulent isogenic morphotypes produced similar amounts of hemolysin. Two mutants deficient in the production of hemolysin and negative for CHO cell activity were characterized and their virulence for mice was examined. Non‐hemolytic mutants were found to be as virulent as their parent strain. It is concluded that the hemolysin produced by V. vulnificus is not required for the full virulence of this pathogen.