Open AccessSheep antibody response to cell wall antigens expressed in vivo by Pasteurella haemolytica serotype A2
Author(s) -
Donachie William,
Gilmour Neil J.L.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03190.x
Subject(s) - pasteurellosis , biology , microbiology and biotechnology , in vivo , bacterial outer membrane , antigen , pasteurella , antibody , serotype , pasteurella multocida , pasteurellaceae , bacteria , immunology , escherichia coli , biochemistry , gene , antibiotics , genetics , haemophilus influenzae
Cells of Pasteurella haemolytica A2 were harvested directly from the pleural fluid of sheep with pneumonic pasteurellosis and the protein composition of their envelopes was examined by SDS‐PAGE. Several high molecular‐weight proteins expressed in pleural fluid were absent when the same isolate was grown in nutrient broth, but were produced during culture in iron restricted media. When the cell wall envelopes were examined by immunoblotting with sera and lung washes of lambs recovering from pneumonic pasteurellosis, antibodies to the major outer membrane proteins, the iron‐regulated proteins and one other ‘in vivo’‐expressed antigen were present. These results have implications for the formulation of effective vaccines against pasteurellosis in sheep.