Open AccessNonfimbrial blood group N‐specific adhesin (NFA‐3) from Escherichia coli O20: KX104: H − , causing systemic infection
Author(s) -
Grünberg J.,
Perry R.,
Hoschützky H.,
Jann B.,
Jann K.,
Goldhar J.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03185.x
Subject(s) - bacterial adhesin , escherichia coli , microbiology and biotechnology , enterobacteriaceae , chemistry , biology , biochemistry , gene
From unfimbriated Escherichia coli O20:KX104: H − , which caused systemic infection in man, and adhesin (NFA‐3) was isolated by extraction from the bacteria in phosphate buffered saline at 70°C. It was purified by ammonium sulfate precipitation and anion exchange chromatography in the presence of urea. The adhesin had an apparent molecular weight in excess to 10 6 daltons and consisted of 17.500‐d peptide subunits. It had an isoelectric point of 3.9 and contained one cysteine and no methionine. In haemagglutination assays and adhesion immunoassays, NFA‐3 showed a preferred specificity for blood group N.