Open AccessCharacterization of a chromosomal mutant that blocks hemolysin excretion in Escherichia coli
Author(s) -
Muñoa F.,
Hacker J.,
Juárez A.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03171.x
Subject(s) - periplasmic space , escherichia coli , mutant , hemolysin , biology , bacterial outer membrane , mutation , bacterial adhesin , enterobacteriaceae , microbiology and biotechnology , genetics , gene , virulence
We analyzed an Escherichia coli strain which harbours a chromosomal mutation that blocks the hemolysin excretion. Compartmentation studies showed that hemolysin accumulates in the cytoplasm and not in the periplasm. The mutation did not affect the SDS‐PAGE protein pattern of the outer membrane, although some alterations were apparent in the periplasmic protein pattern. The mutant strain, E. coli Hsb‐1 also failed to export a cloned fimbrial adhesin. The mutation maps in the min. 3.5 of the E. coli genetic map.