Open AccessPurification and properties of a site‐specific restriction endonuclease Aaa I from Acetobacter aceti subsp. aceti No. 1023
Author(s) -
Tagami Haruko,
Tayama Kenji,
Tohyama Tohru,
Fukaya Masahiro,
Okumura Hajime,
Kawamura Yoshiya,
Horinouchi Sueharu,
Beppu Teruhiko
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03170.x
Subject(s) - isoschizomer , restriction enzyme , escherichia coli , xanthomonas , acetobacter , chemistry , biology , biochemistry , dna , fermentation , gene , gene expression , dna methylation
A type II restriction endonuclease, named Aaa I, was purified from Acetobacter aceti subsp. aceti No. 1023. The optimum pH and temperature were determined to be 8.5 and 37°C, respectively. The enzyme activity was stimulated by the addition of either NaCl or KCl and their optimum concentrations were 100 mM for both cations. Aaa I recognized the hexanucleotide sequence and cleaved it at the positions indicated by the arrows. Aaa I was an isoschizomer of Xma III from Xanthomonas malvacaerum and Eco 52I from Escherichia coli .