Open AccessDetection of sequences homologous to the highly‐conserved HSP70 gene of eukaryotes in thermophilic eubacteria and archaebacteria
Author(s) -
Lambert Emma,
Cowan Don,
Piper Peter W.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03169.x
Subject(s) - thermophile , biology , homology (biology) , gene , genetics , saccharomyces cerevisiae , thermus , nucleic acid sequence , escherichia coli , thermus thermophilus , heat shock protein , conserved sequence , bacteria , peptide sequence
Abstract The HSP70 genes of eukaryotes show up to 50% nucleotide sequence homology to the dna K gene of Escherichia coli . This extreme structure conservation implies conservation of a function that may be needed by all cells, suggesting that other bacteria may have sequences related to HSP70 and dna K. Amongst other functions, HSP70‐like proteins may act to limit thermal protein denaturation. In this study DNA isolated from thermophilic archaebacteria (from the family Desulfurococcus ) and thermophilic eubacteria (from the families Bacillus and Thermus ) was probed with sequences from a heat shock inducible HSP70 gene of the yeast Saccharomyces cerevisiae . Hybridization was detected under conditions of low stringency, indicating the existence of HSP70‐related sequences in the thermophilic bacteria studied.