
Isolation and partial characterization of phenolate siderophore from Rhizobium leguminosarum IARI 102
Author(s) -
Patel H.N.,
Chakraborty R.N.,
Desai S.B.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03164.x
Subject(s) - siderophore , rhizobium leguminosarum , amino acid , rhizobium , chemistry , bacteria , yield (engineering) , microbiology and biotechnology , biology , biochemistry , rhizobiaceae , symbiosis , genetics , materials science , metallurgy
Rhizobium leguminosarum IARI 102 produced 2,3‐dihydroxy benzoic acid, a type of phenolate siderophore, under iron‐starved conditions. Hydroxamic acids were not detected. Maximum production of siderophore was found at 26 h of growth in a chemically defined medium at 28°C with shaking. Threonine was detected as the amino acid conjugate of the siderophore. Addition of Fe 3+ to the culture medium increased the growth yield significantly, but depressed the production of the iron chelating compound.