Open AccessRapid small‐scale DNA isolation from filamentous cyanobacteria
Author(s) -
Smoker Jamesina A.,
Barnum Susan R.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03161.x
Subject(s) - cyanobacteria , electroelution , restriction enzyme , biology , dna , isolation (microbiology) , agarose , plasmid , plasmid preparation , microbiology and biotechnology , genetics , bacteria , biochemistry , enzyme , polyacrylamide gel electrophoresis , pbr322
A rapid small‐scale DNA isolation procedure is described for the filamentous cyanobacteria, which yields enough chromosomal and plasmid DNA for restriction endonuclease digestions, Souther hybridizations, and electroelution from gels for further manipulation. DNA from seven strains of cyanobacteria were isolated and analyzed on agarose gels.