Open AccessTransformation of group A streptococci by electroporation
Author(s) -
Suvorov Alexander,
Kok Jan,
Venema Gerard
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03156.x
Subject(s) - electroporation , plasmid , transformation (genetics) , streptococcus pyogenes , lactococcus lactis , lactococcus , escherichia coli , microbiology and biotechnology , biology , streptococcaceae , dna , streptococcus , bacteria , genetics , gene , staphylococcus aureus , lactic acid
The introduction, via electroporation, of free plasmid DNA into three strains of Streptococcus pyogenes is described. The method is very simple and rapid and efficiencies vary from 1 × 10 3 to 4 × 10 4 per μg of DNA. The method was also used to introduce an integrative plasmid and transformants were obtained, albeit at a somewhat lower frequency (2 × 10 2 ). Some of the plasmids used in this study are derivatives of the Lactococcus lactis subsp. cremoris Wg2 plasmid pWV01. These broad host range vectors replicate in Gram‐positives as well as Gram‐negatives (viz. Escherichia coli ). Here we show that they also replicate in S. pyogenes and S. sanguis .