Open AccessImprovements in the transformation of Bacillus subtilis protoplasts with plasmid DNA
Author(s) -
Rubinstein C.P.,
Moratinos L.C.,
Coso O.A.,
SánchezRivas C.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03151.x
Subject(s) - bacillus subtilis , plasmid , protoplast , transformation (genetics) , bacillaceae , dna , microbiology and biotechnology , bacillales , biology , plasmid preparation , genetics , bacteria , chemistry , gene , pbr322
The transformation system currently used for Bacillus subtilis protoplasts has been improved. Special emphasis was made on three parameters of practical importance: (a) conditions for direct selection of transformants, (b) optimization of the transformation system for Rec − mutants, and (c) conservation of protoplast suspensions for further use. Selective regeneration was efficiently achieved for kanamycin or neomycin. Chloramphenicol, tetracycline and erythromycin were only expressed when low concentrations of the antibiotics were used to select transformants during regeneration.