Open AccessA catalogue of Clostridium thermocellum endoglucanase, β‐glucosidase and xylanase genes cloned in Escherichia coli
Author(s) -
Hazlewood Geoffrey P.,
Romaniec Marek P.M.,
Davidson Keith,
Grépinet Olivier,
Béguin Pierre,
Millet Jacqueline,
Raynaud Odette,
Aubert JeanPaul
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb03002.x
Subject(s) - clostridium thermocellum , xylanase , gene , cellulase , biology , restriction enzyme , escherichia coli , genetics , restriction map , microbiology and biotechnology , enzyme , biochemistry , plasmid
Two independent collections of clones containing Clostridium thermocellum genes involved in cellulose have been previously obtained at IAPGR, Cambridge, and at the Pasteur Institute, Paris. The two collections were compared for cross‐hybridization, restriction maps and enzyme phenotypes. Truly distinct genes were one β‐glucosidase gene, two xylanase genes, and fifteen endogluconase genes. Two of the cloned fragments contained extraneous DNA which was absent from their respective counterparts isolated in the other collection. The dicrepancies resulted from in vivo rearrangements which had occurred in either of the C. thermocellum NCIB 10682 stocks used to generate the two gene banks.