Open AccessMonoclonal antibodies against O and K antigens of uropathogenic Escherichia coli O4 : K12 : H − as opsonins
Author(s) -
Abe Chiyoji,
Schmitz Sylvia,
Jann Barbara,
Jann Klaus
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02988.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , epitope , escherichia coli , antibody , opsonin , antigen , phagocytosis , biology , lipopolysaccharide , chemistry , biochemistry , immunology , gene
Monoclonal antibodies of subclasses IgG1 and IgG2b and specific for the O4 antigen of Escherichia coli 20025 (O4 : K12 : H − ) and the capsular K12 polysaccharide of the same strain (IgM) were obtained with the hybridoma technique using spleen cells from Balb/c mice, immunized with a crude bacterial extract, and Sp2/O‐Ag8 myeloma cells. The anti‐O4 antibodies reacted exclusively with the O4 lipopolysaccharide and not with those from serologically O‐cross reactive E. coli . The anti‐K12 antibodies recognized as epitope (part of) the KDO moiety of the capsular K12 polysaccharide. Not only anti‐K12, but also anti‐O4 antibodies effectively phagoopsonized encapsulated E. coli 20025. The opsonized bacteria were killed in subsequent in vitro phagocytosis by human leokocytes in the presence of human serum complement.