Open AccessEffect of cyanophage N‐1 development on nitrogen metabolism of cyanobacterium Nostoc muscorum
Author(s) -
Kashyap A.K.,
Rai A.N.,
Singh Surendra
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02986.x
Subject(s) - nitrogenase , cyanobacteria , transaminase , diazotroph , biology , glutamine synthetase , biochemistry , metabolism , glutamate synthase , glutamine , nitrogen fixation , microbiology and biotechnology , enzyme , amino acid , bacteria , genetics
The impact of cyanophage N‐1 development on nitrogenase, glutamine synthetase (GS) and aminotransferases activities in the diazotrophic cyanobacterium Nostoc muscorum was investigated during its latent period. The nitrogenase activity was inhibited after 2 h of infection, suggesting that phage development does not require the product of nitrogenase activity. GS activity was not inhibited until 4 h of infection; however, a decline in activity was subsequently observed. Glutamate oxaloacetate transaminase was inhibited after 1 h of infection and no activity was detectable during the entire latent period. In contrast, glutamate pyruvate transaminase activity increased 2‐fold by 4 h of infection and remained higher than the background level until the end of the latent period. The results suggested that under nitrogen fixing conditions, N‐1 multiplication proceeds in the absence of nitrogen fixation and that the metabolism of amino acids is altered in favour of phage multiplication.