Open AccessMonoclonal antibodies to Streptococcus mutans serotype f polysaccharide cross‐react with the ‘mutans group’ of oral streptococci
Author(s) -
Scholler M.,
Fargeas C.,
Pini A.,
Klein J.P.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02982.x
Subject(s) - streptococcus mutans , epitope , serotype , monoclonal antibody , microbiology and biotechnology , polysaccharide , biology , antibody , saliva , splenocyte , chemistry , bacteria , biochemistry , immunology , genetics
Eleven stable clones producing monoclonal antibodies (MAbs) against serotype polysaccharide f were generated from rat IR 983F myeloma cells and splenocytes from rats immunized with formalin‐killed whole Streptococcus mutants OMZ 175. These MAbs were IgG 2b, they had different affinities towards polysaccharide f and all but three were able to inhibit saliva binding to S. mutants OMZ 175 cells. Two specificities were recognized on S. mutans serotype f polysaccharide. One MAb (K24) recognized an epitope on the poly‐rhamnose core, as determined by competitive ELISA using different sugars. The ten other MAbs recognized an epitope which seems principally formed of glucose subunits. Purified polysaccharides from other ‘mutans streptococci’ were able, to different extents, to inhibit the binding of the MAbs to polysaccharide f, confirming the presence of common epitopes in the different strains.