Open AccessIdentification of l ‐iduronic acid as a constituent of the major extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain X6C61
Author(s) -
Stack Robert J.,
Plattner Ronald D.,
Cote Gregory L.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02957.x
Subject(s) - rhamnose , chemistry , chromatography , sugar , galactose , polysaccharide , xylose , mannose , arabinose , thin layer chromatography , iduronic acid , biochemistry , glucuronic acid , fermentation
Butyrivibrio fibrisolvens strain X6C61 produces two extracellular polysaccharides (EPS‐I and EPS‐ II) separable by anion‐exchange chromatography. The neutral sugar constituents of EPS‐I were identified by gas‐liquid chromatography (GLC) as the alditol acetates of rhamnose, mannose, galactose, glucose, and an unidentified component. These results were confirmed using thin‐layer chromatography (TLC). Neutral sugar analysis of EPS‐II, which eluted from DEAE‐Sephadex at 0.4 M NaCl, yielded the alditol acetates of rhamnose, galactose, glucose, and idose. However, idose was not found when hydrolysates of EPS‐II were analysed by TLC. Further investigations showed that the iditol hexaacetate detected via GLC was an artifact of the commonly‐used procedures for neutral sugar analysis. This compound was instead generated from l ‐iduronic acid, as shown by GLC‐MS studies.