Open AccessExpression of Escherichia coli PhoE protein in avirulent Salmonella typhimurium aroA and galE strains
Author(s) -
Agterberg Marja,
Fransen Rene,
Tommassen Jan
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02953.x
Subject(s) - bacterial outer membrane , epitope , aroa , salmonella , escherichia coli , biology , microbiology and biotechnology , antigen , enterobacteriaceae , virology , porin , strain (injury) , bacteria , biochemistry , genetics , gene , anatomy
Escherichia coli K‐12 PhoE protein is found to be normally expressed and incorporated into the outer membrane of two avirulent Salmonella typhimurium strains, G30 and SH aroA . A hybrid protein which contains an insertion of an antigenic epitope of VP1 protein of foot‐and‐mouth disease virus into the PhoE protein, was also normally assembled into the Salmonella outer membranes. In the case of the G30 stain, which carries a galE mutation, the inserted epitope is accessible to antibodies in intact cells. In contrast, the epitope is less accessible in the case of the SH aroA strain, probably due to the shielding effect of the O‐antigen in this strain.