Open AccessA plasmid vector for the selection and study of transcription promoters in Azospirillum brasilense
Author(s) -
Fani Renato,
Bazzicalupo Marco,
Ricci Fiamma,
Schipani Concetta,
Polsinelli Mario
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02950.x
Subject(s) - azospirillum brasilense , plasmid , promoter , biology , pseudomonas putida , escherichia coli , cloning vector , multiple cloning site , genetics , microbiology and biotechnology , gene , vector (molecular biology) , microbial inoculant , recombinant dna , bacteria , gene expression
The construction of the plasmid pAF300, which contains the promoterless gene for chloramphenicol resistance fused to the broad host range conjugative vector pRK290, is described. Plasmid pAF300 could be used to identify transcription promoters by inserting DNA sequences in two cloning sites, Hin dIII and Pst I. The vector efficiency was proved by cloning promoter sequences from Escherichia coli and Azospirillum brasilense which conferred chloramphenicol resistance to E. coli, A. brasilense and Pseudomonas putida .