Open AccessIntracellular precursors of endo‐β‐1,4‐glucanase in Trichoderma reesei
Author(s) -
Messner Robert,
Kubicek Christian P.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02943.x
Subject(s) - trichoderma reesei , intracellular , catabolite repression , extracellular , biochemistry , gel electrophoresis , chemistry , secretion , sodium dodecyl sulfate , glucanase , glycosylation , cellulase , enzyme , gene , mutant
Sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis (PAGE) followed by immunoblotting was employed to detect intracellular precursors of endo‐β‐1,4‐glucanases (EGs) in Trichoderma reesei QM9414 under conditions of de novo induction by sophorose and de novo carbon catabolite derepression by lactose. Secretion of EGs was always preceded by intracellular accumulation of lower M r precursors, which became processed to larger M r forms immediately prior to their extracellular appearance. Treatment of the larger M r forms with α‐mannosidase converted them to forms with the same M r as the smaller forms, whereas Endo H treatment was without effect. These results are consistent with a requirement of O ‐linked glycosylation for secretion of EGs by T. reesei .