Open AccessProduction of an enterotoxin different from toxin A by Clostridium difficile
Author(s) -
Giuliano Marina,
Piemonte Fiorella,
Mastrantonio Gianfrilli Paola
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02936.x
Subject(s) - enterotoxin , toxin , clostridium difficile toxin a , clostridium difficile , microbiology and biotechnology , clostridium , gel electrophoresis , polyacrylamide gel electrophoresis , clostridiaceae , chemistry , biology , bacteria , chromatography , biochemistry , escherichia coli , enzyme , antibiotics , gene , genetics
Clostridium difficile has been demonstrated to produce at least two toxins: an enterotoxin (toxin A) which elicits haemorrhagoc fluid accumulation in the rabbit ileal loop (RIL) test and a potent cytotoxin (toxin B). We report the isolation of an enterotoxic factor inducing a positive response in the RIL test without haemorrhage. This factor was separated by ion‐exchange chromatography and its molecular weight, as estimated by SDS‐polyacrylamide gel electrophoresis, was about 45 000.