Open AccessPermeability of gentamicin into the inside of Bacillus subtilis spores
Author(s) -
Tochikubo Kunio,
Kozuka Satoshi,
Yasuda Yoko
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02926.x
Subject(s) - bacillus subtilis , spore , gentamicin , colloid , immunoelectron microscopy , colloidal gold , microbiology and biotechnology , permeability (electromagnetism) , penetration (warfare) , biophysics , chemistry , membrane , biology , bacteria , antibiotics , nanotechnology , materials science , biochemistry , nanoparticle , antibody , immunology , genetics , engineering , operations research
The penetration of gentamicin into the inside of Bacillus subtilis spores was examined by an immunoelectron microscopy method with colloidal gold‐immunoglobulin G complex. The colloidal gold particles were located mainly in the coat regions of spores and were not observed in the cortex or core regions. This result suggests the existence of an outer membrane inside the coat region as the primary permeability barrier to gentamicin.