Open AccessCloning and sequencing of genes encoding the beta subunits of the ATP‐synthases from Enterobacter aerogenes and Flavobacterium ferrugineum
Author(s) -
Amann Rudolf,
Sostak Petra,
Ludwig Wolfgang,
Schleifer Karl Heinz
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02919.x
Subject(s) - enterobacter aerogenes , escherichia coli , biology , homology (biology) , protein subunit , gene , flavobacterium , genetics , amino acid , peptide sequence , cloning (programming) , biochemistry , microbiology and biotechnology , bacteria , pseudomonas , computer science , programming language
The genes encoding the β‐subunit of the ATPase from Enterobacter aerogenes and Flavobacterium ferrugineum were cloned and their sequences determined. The predicted amino acid sequences were compared with the corresponding proteins from other eubacteria. Homology values of 58–98% confirmed the highly conserved character of the ATPase β‐subunit. The enterobacterial ( Escherichia coli, E. aerogenes ) β‐subunits represent the shortest sequences, whereas the corresponding F. ferrugineum protein exhibits an additional 33 amino acid residues as insertions at three different locations.