Open AccessIsolation and characterisation of plasmids from a wild‐type strain of the extremely thermophilic eubacterium Thermus aquaticus
Author(s) -
Kröger B.,
Specht T.,
Lurz R.,
Ulbrich N.,
Erdmann V.A.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02912.x
Subject(s) - thermus , plasmid , eubacterium , thermus aquaticus , thermophile , biology , microbiology and biotechnology , gel electrophoresis , strain (injury) , thermus thermophilus , escherichia coli , bacteria , dna , genetics , gene , anatomy
We examined the extremely thermophilic Gram‐negative eubacterium Thermus aquaticus for the presence of plasmid DNA, and detected 5 species. The sizes were determined by electron microscopy and gel electrophoresis. The plasmids, pTA 1‐pTA 5, were of respective length: 13.5, 12.4, 11.2, 9.6 and 8.3 kb. Clones were isolated which differed in plasmid distribution. No correlation could be observed between the phenotype or growth behaviour and the presence of plasmids. The function of the plasmid structures still needs to be elucidated.