Open AccessProtein antigens of Staphylococcus epidermidis grown under iron‐restricted conditions in human peritoneal dialysate
Author(s) -
Williams Paul,
Denyer Stephen P.,
Finch Roger G.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02906.x
Subject(s) - staphylococcus epidermidis , microbiology and biotechnology , antigen , chemistry , staphylococcus aureus , staphylococcus , bacteria , biology , immunology , genetics
Comparison of the whole cell protein profiles of Staphylococcus epidermidis grown in pooled human peritoneal dialysate (HPD) with those of cells grown in nutrient broth (NB) revealed proteins of 27, 39, 45, 54 and 98 kDa which were absent or poorly expressed in NB‐grown cells. IgG, but not transferrin, was detected bound to the surface of bacteria grown in HPD. Immunoblotting experiments revealed that IgG antibodies present in pooled HPD recognised staphylococcal protein antigens of 16, 27, 35, 39, 45, 54 and 98 kDa. The 16‐, 35‐ and 39‐kDa antigens which were associated with the cytoplasmic membrane were repressed following growth in NB or in HPD supplemented with excess iron.