Open AccessIncreased susceptibility of plasmid pBR322 in Escherichia coli HB101 to curing agents after pretreatment with hexammine ruthenium (III) chloride
Author(s) -
Durga Rama,
Sridhar Padma,
Polasa H.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02905.x
Subject(s) - plasmid , pbr322 , ruthenium , acridine orange , curing (chemistry) , escherichia coli , chemistry , nuclear chemistry , chloride , catalysis , microbiology and biotechnology , biology , dna , biochemistry , polymer chemistry , organic chemistry , gene , apoptosis
In our earlier studies, hexammine ruthenium (III) chloride (HRC) was found to eliminate the pBR322 plasmid from E. coli HB101 with 100% frequency. However, this plasmid was not eliminated by other known curing agents, such as acridine orange, sodium dodecyl sulphate (SDS), rifampicin or temperature conditions. In our present communication, we report the sensitivity of a variant of plasmid pBR322 to curing agents which were not effective on the parent plasmid.