Open AccessAssay‐IgG to Campylobacter pylori in histological chronic and biopsy Campylobacter positive gastritis
Author(s) -
Bohemen Ch.G.,
Langenberg M.L.,
Rauws E.A.J.,
Weterings E.,
Zanen H.C.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02822.x
Subject(s) - campylobacter , chronic gastritis , gastritis , titer , antibody , medicine , helicobacter pylori , gastroenterology , immunology , biology , bacteria , genetics
A convenient enzyme‐linked immunoabsorbent assay (ELISA) to titrate serum immunoglobin G to Campylobacter pylori (Cp) is described. It was found that 80% of the serum samples obtained from patients with Cp‐associated chronic gastritis ( N = 476) displayed statistically raised IgG anti‐Cp, as compared to healthy volunteer controls ( N = 24). Titers in patient sera were statistically higher than the average control value plus twice the standard deviation, thus supporting Cp involvement in these gastric diseases. Also, ELISA inhibition experiments suggested that antigenic variation among various Cp isolates is likely, thus indicating the need for subgrouping of Cp. We note the usefulness of the above IgG anti‐Cp ELISA for sero‐epidemiological studies of Cp associated chronic gastritis, especially those tracing the source of Cp infections and those determining potentially disease related Cp subgroups.