Open AccessCharacterisation of the cell envelope of Treponema hyodysenteriae
Author(s) -
Chatfield Steven N.,
Fernie David S.,
Beesley Julian,
Penn Charles,
Dougan Gordon
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02819.x
Subject(s) - treponema , cell envelope , antigen , microbiology and biotechnology , biology , cell , antibody , immune system , strain (injury) , cell wall , chemistry , virology , biochemistry , immunology , escherichia coli , gene , syphilis , anatomy , human immunodeficiency virus (hiv)
Cell envelope preparations of Treponema hyodysenteriae (strain CN 8368) were examined using biochemical and immunochemical methods. Several major polypeptides were detected with molecular weight between 24‐kDa and 45‐kDa. The majority of these polypeptides were recognised by serum from a pig vaccinated with an experimental whole‐cell T. hyodysenteriae vaccine and hyperimmune anti‐ T. hyodysenteriae rabbit sera. Immune electron microscopy confirmed that the major antigens detected were associated with the cell envelope. Triton X‐100, in the presence of EDTA, completely solubilised a polypeptide with an approximate molecular weight of 36‐kDa. Antibodies to this polypeptide were not absorbed by whole T. innocens cells.