Open AccessCellular and extracellular localization of endocellulase in Trichoderma reesei
Author(s) -
Sprey Benno
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02816.x
Subject(s) - trichoderma reesei , immunogold labelling , cellulase , cell wall , cellulose , extracellular , isoelectric focusing , biochemistry , microbiology and biotechnology , chemistry , trichoderma viride , isoelectric point , biology , antibody , enzyme , food science , immunology
An endocellulase (1,4‐β‐ d ‐glucan 4‐glucanohydrolase, EC 3.2.1.4) was purified by preparative isoelectric focusing from culture fluids of Trichoderma reesei QM 9414 grown on cellulose. Its properties were studied by affinity titration curves and immunoelectrophoresis. FITC‐labeled protein A‐antibody was used to document its occurrence in cellulose and in fungal cell walls. Immunogold electron microscopy served to detect endocellulose sites within the outer exopolysaccharide layer of the fungal cell wall.