Open AccessInduction of interferon messenger RNA in human lymphoblastoid cells (Namalva)
Author(s) -
Massoud Mahmoudi,
Vela G.R.,
Bollon Arthur P.,
Osther Kurt
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02809.x
Subject(s) - sendai virus , messenger rna , interferon , lymphoblast , northern blot , microbiology and biotechnology , biology , titer , virology , rna , virus , cell culture , gene , biochemistry , genetics
Human B lymphoblastoid cells Namalva were induced by Sendai virus and the levels of α‐Interferon (IFN‐α)mRNA, and IFN‐α protein were measured. The level of IFN‐αmRNA reached a maximum level 6 h after induction and then slowly declined. The interferon titer reached its maximum level 18 to 24 h after induction. Northern blot analysis of induced Namalva cell poly(A) + mRNA with an interferon probe revealed a transcript of 1.2 kb. No IFN‐α mRNA was detected in uninduced Namalva cells under the conditions used. This study adds to our understanding of the events which lead to interferon production in vitro.