Open AccessBacterial catabolism of sulfanilic acid via catechol‐4‐sulfonic acid
Author(s) -
Feigel Burkhard J.,
Knackmuss HansJoachim
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02807.x
Subject(s) - sulfanilic acid , catechol , sulfonic acid , metabolite , chemistry , catabolism , strain (injury) , biochemistry , chromatography , nuclear chemistry , organic chemistry , stereochemistry , metabolism , biology , anatomy
A sulfanilic acid (4‐aminobenzenesulfonic acid) degrading culture consisting of two strains (strain S1 and S2), was studied. Only strain S1 was able to attack sulfanilic acid. When strain S1 was cultavated in a mineral medium with sulfanilic acid an intensive violet colour was observed. The accumulating metabolite was isolated from the culture supernatant. By comparison with an authentic compound the metabolite was identified as catechol‐4‐sulfonic acid by thin layer and high performance liquid chromatography and by UV‐ and H‐NMR spectroscopy. The occurrence of catechol‐4‐sulfonic acid indicates that there is no release of the sulfonic group before ring cleavage.