Open AccessDNA‐methyltransferase activities in Streptomyces antibioticus
Author(s) -
Barbes Covadonga,
Hardisson Carlos,
Novella Isabel S.,
Jesus Yebra M.,
Sanchez Jesus
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02798.x
Subject(s) - methyltransferase , enzyme , mycelium , dna , biochemistry , streptomyces , streptomycetaceae , biology , actinomycetales , methylation , genetics , bacteria , botany
Several DNA methyltransferases have been detected in Streptomyces antibioticus ETHZ 7451. One of these enzymes was purified and partially characterized from crude extracts of Streptomyces antibioticus ETHZ 7451. This enzyme only methylates the adenine residues of DNA. However, λ and pBR322 DNAS were not protected ‘in vitro’ with the methylase, indicating that the enzyme does not seem to form part of a restriction‐modification system. Remarkably, the efficiency of the enzyme is significantly higher on the DNA isolated from aerial mycelium than from substrate mycelium in S. antibioticus .